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- W2357721916 abstract "Objective To investigate the feasibility of contrast enhanced ultrasound (CEU) with VEGFR2-targeted microbubbles (MBv) to assessment tumor angiogenesis. Methods MBv was prepared by biotin-avidin conjugation method. The conjugation was demonstrated by immunofluorescence. H22 tumor models in mice were established and underwent CEU examination with MBv or control microbubbles (MBc). Sonograms were analyzed by calculation of the normalized video intensity (VI), and time-intensity curve (TIC) was depicted according to VI changes. Tumor samples were harvested for analysis of VEGFR2 expressions by immunohistochemistry. Results MBv was demonstrated bounding with anti-VEGFR2 antibody effectively. TIC showed that the time to maximum echogenicity was earlier, peak echogenicity was higher, and the time to wash out was longer in MBv contrast group compared with that in MBc group. Immunohistochemistry showed VEGFR2 was highly expressed in novel vessel walls. Conclusion Tumor angiogenesis can be well evaluated using CEU with MBv. MBv can be regarded as a potential tumor targeted contrast agent." @default.
- W2357721916 created "2016-06-24" @default.
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- W2357721916 date "2009-01-01" @default.
- W2357721916 modified "2023-09-23" @default.
- W2357721916 title "Assessment of tumor angiogenesis using contrast enhanced ultrasound with VEGFR2-targeted microbubbles" @default.
- W2357721916 hasPublicationYear "2009" @default.
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