Matches in SemOpenAlex for { <https://semopenalex.org/work/W2357732711> ?p ?o ?g. }
Showing items 1 to 81 of
81
with 100 items per page.
- W2357732711 endingPage "2160" @default.
- W2357732711 startingPage "2156" @default.
- W2357732711 abstract "OBJECTIVE: To establish a prediction method for the refolding of inclusion bodies and classify refolding types of different inclusion bodies directly from their primary structure to improve the efficiency of high throughput refolding process. METHODS: Forty-three recombinant proteins performing important biological functions were expressed in E. coli. The probability of forming inclusion bodies of these proteins was predicted using Harrison's two parameter prediction model based on the proteins' amino acid composition. Subsequently, the proteins from the inclusion bodies were refolded using a double denaturation method that involved washing and denaturation in GdnHCl solution followed by denaturation in Urea solution and refolding through dilution. RESULTS: All the proteins were detected in the form of inclusion bodies using SDS-PAGE method. The proteins were divided into two types according to the results of both solubility prediction and refolding experiments. Fourteen proteins were predicted to have the dependency of soluble expression. The refolding yields of these inclusion bodies were up to 70%. Twenty-nine proteins were predicted to have the high dependency of insoluble expression, and their refolding yields could be higher than 70% and lower than 60%. Comparison of the characteristics between the proteins with high and low refolding yields showed that the theoretical pI was significantly different (P<0.05). CONCLUSIONS: Harrison's two parameter prediction model has the value for potential application in classification of the inclusion bodies and prediction of solubility of proteins refolded from different inclusion bodies. This a novel method enhances the efficiency of high throughput refolding of inclusion bodies, and suggests that the theoretical pI of the proteins is an important parameter in the prediction of refolding yields." @default.
- W2357732711 created "2016-06-24" @default.
- W2357732711 creator A5001591660 @default.
- W2357732711 creator A5062097482 @default.
- W2357732711 creator A5065682688 @default.
- W2357732711 creator A5083903809 @default.
- W2357732711 creator A5088174174 @default.
- W2357732711 creator A5090656526 @default.
- W2357732711 date "2009-11-01" @default.
- W2357732711 modified "2023-09-22" @default.
- W2357732711 title "Application of a prediction model in inclusion body refolding" @default.
- W2357732711 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19923054" @default.
- W2357732711 hasPublicationYear "2009" @default.
- W2357732711 type Work @default.
- W2357732711 sameAs 2357732711 @default.
- W2357732711 citedByCount "0" @default.
- W2357732711 crossrefType "journal-article" @default.
- W2357732711 hasAuthorship W2357732711A5001591660 @default.
- W2357732711 hasAuthorship W2357732711A5062097482 @default.
- W2357732711 hasAuthorship W2357732711A5065682688 @default.
- W2357732711 hasAuthorship W2357732711A5083903809 @default.
- W2357732711 hasAuthorship W2357732711A5088174174 @default.
- W2357732711 hasAuthorship W2357732711A5090656526 @default.
- W2357732711 hasConcept C104317684 @default.
- W2357732711 hasConcept C109359841 @default.
- W2357732711 hasConcept C13965031 @default.
- W2357732711 hasConcept C155574463 @default.
- W2357732711 hasConcept C178790620 @default.
- W2357732711 hasConcept C185592680 @default.
- W2357732711 hasConcept C199289684 @default.
- W2357732711 hasConcept C203750385 @default.
- W2357732711 hasConcept C204328495 @default.
- W2357732711 hasConcept C2776923230 @default.
- W2357732711 hasConcept C40767141 @default.
- W2357732711 hasConcept C43617362 @default.
- W2357732711 hasConcept C55493867 @default.
- W2357732711 hasConceptScore W2357732711C104317684 @default.
- W2357732711 hasConceptScore W2357732711C109359841 @default.
- W2357732711 hasConceptScore W2357732711C13965031 @default.
- W2357732711 hasConceptScore W2357732711C155574463 @default.
- W2357732711 hasConceptScore W2357732711C178790620 @default.
- W2357732711 hasConceptScore W2357732711C185592680 @default.
- W2357732711 hasConceptScore W2357732711C199289684 @default.
- W2357732711 hasConceptScore W2357732711C203750385 @default.
- W2357732711 hasConceptScore W2357732711C204328495 @default.
- W2357732711 hasConceptScore W2357732711C2776923230 @default.
- W2357732711 hasConceptScore W2357732711C40767141 @default.
- W2357732711 hasConceptScore W2357732711C43617362 @default.
- W2357732711 hasConceptScore W2357732711C55493867 @default.
- W2357732711 hasIssue "11" @default.
- W2357732711 hasLocation W23577327111 @default.
- W2357732711 hasLocation W23577327112 @default.
- W2357732711 hasOpenAccess W2357732711 @default.
- W2357732711 hasPrimaryLocation W23577327111 @default.
- W2357732711 hasRelatedWork W159157277 @default.
- W2357732711 hasRelatedWork W1939054711 @default.
- W2357732711 hasRelatedWork W2020212788 @default.
- W2357732711 hasRelatedWork W2125537720 @default.
- W2357732711 hasRelatedWork W2131291940 @default.
- W2357732711 hasRelatedWork W2138816428 @default.
- W2357732711 hasRelatedWork W2153753900 @default.
- W2357732711 hasRelatedWork W2155473277 @default.
- W2357732711 hasRelatedWork W2312418504 @default.
- W2357732711 hasRelatedWork W2349574078 @default.
- W2357732711 hasRelatedWork W2361686274 @default.
- W2357732711 hasRelatedWork W2364757915 @default.
- W2357732711 hasRelatedWork W2384944396 @default.
- W2357732711 hasRelatedWork W2386779908 @default.
- W2357732711 hasRelatedWork W2389006370 @default.
- W2357732711 hasRelatedWork W2771747150 @default.
- W2357732711 hasRelatedWork W2954938277 @default.
- W2357732711 hasRelatedWork W3046273814 @default.
- W2357732711 hasRelatedWork W86724569 @default.
- W2357732711 hasRelatedWork W2044526622 @default.
- W2357732711 hasVolume "29" @default.
- W2357732711 isParatext "false" @default.
- W2357732711 isRetracted "false" @default.
- W2357732711 magId "2357732711" @default.
- W2357732711 workType "article" @default.