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- W2357736139 abstract "Objective To construct a vector of recombinant adeno-associated virus(AAV) containing the fusion gene hTRX-PR39,and explore the role of fusion gene hTRX-PR39 as a target gene in the treatment of cerebral ischemic disease.Methods The constructed fusion gene hTRX-PR39 was inserted into the EcoRI-BamHI site of vector plasmid pSSCMV,and the vector of hTRX-PR39 recombinant AAV was constructed.The recombinant AAV stock was packaged.Renal embryo 293 cells were co-transfected with the recombinant AAV vector of plasmid pSSCMV/hTRX-PR39,packaging plasmid pAAV/Ad and helper adenovirus plasmid pFG140.Recombinant AAV was produced by homologous recombination of the 3 plasmids in renal embryo 293 cells and its titer was measured by quantitative dot blot hybridization.Results The vector of hTRX-PR39 recombinant AAV was successfully constructed.High titer of recombinant AAV was obtained by homologous recombination in renal embryo 293 cells(3.46×1012-3.46×1013PFU/mL).Conclusion The recombinant AAV vector encoding the gene hTRX-PR39 was successfully constructed in this study by molecular cloning and in vitro recombination techniques,laying the foundation for further research into genetic therapy of cerebral ischemic disease." @default.
- W2357736139 created "2016-06-24" @default.
- W2357736139 creator A5052800839 @default.
- W2357736139 date "2011-01-01" @default.
- W2357736139 modified "2023-09-23" @default.
- W2357736139 title "Construction of a hTRX-PR39 recombinant adeno-associated virus vector" @default.
- W2357736139 hasPublicationYear "2011" @default.
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