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- W2357753079 abstract "A panel of four hybridoma cell lines secreting specific antibodies to F18ab pili antigens of E.coli were established from three fusions between mouse myeloma Sp2/0-Ag-14 and spleen cells from mice immunized with purified F18ab pili antigens.The titre of their ascitic fluid was 1∶400-1∶(3 200) in direct agglutination(DA).Monoclonal antibodies(McAbs) reacted specifically with F18ab pili from 107/86,while not with F4,F5,F6,F41 adhesins from enterotoxigenic E.coli(ETEC) and Salmonella Spp in direct agglutination test.Western-blot analysis confirmed that 4 McAbs just reacted with (15 000) subunit of F18ab pili from strain 107/86.The golden particles irregularly distributed along the F18ab pili of strain 107/86,when reacted with immunogold labeled 4 McAbs.The McAbs have been used in DA to detect F18ab pili antigens of 11 isolates from swines with edema disease.8 out of 11 were positive,and all of their O serogroups were O_(139).The results suggested that F18ab may be the common pili on E.coli isolated from swine with edema disease." @default.
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- W2357753079 date "2004-01-01" @default.
- W2357753079 modified "2023-09-25" @default.
- W2357753079 title "Development and Potential Application of Monoclonal Antibodies against F18ab Pili of Escherichia coli Caused Swine Edema Disease" @default.
- W2357753079 hasPublicationYear "2004" @default.
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