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- W2357757670 abstract "Chicken interleukin-2(ChIL-2)is a kind of important cytokine discovered recently.One pair of sepcific primers were designed according to IL-2 gene sequence published by Sundick,et al,IL-2 gene of silken feather Silky chicken was cloned by RT-PCR method and mRNA were extracted from spleen lymphocytes activated by ConA.Sequencing results showed that the gene was 734bp in length,encoding a preprotein of 143aa residues.There were an URT of 17 nucleotides at 5'-terminal of the gene of an UTR of 285 nucleotides along with 4 repetitive sequences of ATTTA at 3' terminal of the gene.Compared with that of leghorn(Kestrel,Obese and SC),the deduced amino acids were predominantly mutated at the region of 28-32.The sequence of this region were entirely conserved between Silky and Kestrel,and also between Obese and SC.Phylogenetic analysis showed there are close relationships between Silky and Kestrel,and also between Obese and SC.Amino acids nutation were found at the region of 133 of Chinese officinal chicken breed-silken feather Silky chicken,which were highly conserved usually,we sepculated that this may be related with its high resistence against sickness." @default.
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- W2357757670 date "2003-01-01" @default.
- W2357757670 modified "2023-09-23" @default.
- W2357757670 title "Cloning and Genetic Analysis of Interleukin 2 Gene in Silken Feather Silky Chicken of Chinese Local Breed" @default.
- W2357757670 hasPublicationYear "2003" @default.
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