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- W2357788014 abstract "BACKGROUNDAIM: To determine whether an overexpression of IP-10by transfection experiments in MA-10cells has any effect on cell growth and progesterone synthesis. MATERIAL AND METHODS: We cloned the complete IP-10cDNA in a mammalian expression vector with CMV promoter,pcDNA3.1D/V5-His-TOPO and transfected MA-10cells.We checked the expression with rat IP-10antibody and V5antibody using Western blotting.The effects of overexpression of IP-10gene on cell growth were checked by [ 3 H]thymidine incorporation experiment.The progesterone synthesis was checked by RIA method. RESULTS: Results showed that IP-10protein secreted in the medium was30～40fold more in the IP-10transfectants over the basal levels as estimated by Western blotting.Transfection of MA-10cells with IP-10decreased8-bromo-cAMP-induced progesterone synthesis from(38.5±1.7)ng/ml(1.5×10 5 cells/ml)of control cells to(23.2±1.5)ng/ml in transfected cells(P0.01).8-bromo-cAMP(0.2mmol/L)induced StAR D1mRNA and decreased30%～40%by transfection with IP-10.Transfection of IP-10gene also significantly decreased insulin-like growth factor(IGF-I,100ng/ml)induced [ 3 H] thymidine incorporation into DNA. CONCLUSION: IP-10inhibits StAR D1expression,decreases progesterone synthesis and inhibits cell proliferation.IP-10can be used as gene therapy for prostate cancer due to its antiangiogenic effects and its inhibitory effects on steroidogenesis." @default.
- W2357788014 created "2016-06-24" @default.
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- W2357788014 date "2005-01-01" @default.
- W2357788014 modified "2023-09-25" @default.
- W2357788014 title "Effects of Overexpression of IP-10Gene on MA-10 Mouse Leydig Tumor Cell Steroidoge-nesis and Cell Proliferation" @default.
- W2357788014 hasPublicationYear "2005" @default.
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