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- W2357789689 abstract "In order to obtain functional genes, a normalized stems cDNA library was constructed from medicinal plant Dendrobium officinale.SMART (switching mechanism at 5' end of RNA transcript) cDNA synthesis combined with DSN (duplex-specific nuclease) normalization was applied to construct the normalized full-length cDNA library of D. officinale.The titer of cDNA library was about 1.3 x 10(6) cfu x mL(-1) and the average insertion size was about 1.5 kb with high recombination rate (93.9%). Random selected 163 positive clones were sequenced at single side. Bio-information analysis indicated that 147 from 150 high-quality unique sequences matched corresponding homologous proteins, and they participated in various biological processes based on GO (gene ontology). There were 8 clones with complete coding sequence, which presumed to be full-length genes.These results showed preliminarily that we successfully constructed a normalized full-length cDNA library of D. officinale which could be used to screen the functional genes related to metabolic pathways of medicinal ingredients." @default.
- W2357789689 created "2016-06-24" @default.
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- W2357789689 date "2013-02-15" @default.
- W2357789689 modified "2023-09-26" @default.
- W2357789689 title "Construction and sequence analysis of a normalized full-length cDNA library of Dendrobium officinale" @default.
- W2357789689 doi "https://doi.org/10.4268/cjcmm20130408" @default.
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