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- W2357802605 abstract "Objective To investigate whether laminin (LN) can affect the expression of matrix metalloproteinase-2 (MMP-2), matrix metalloproteinase-9 (MMP-9) and their mRNA in the human retinoblastoma cell strain. Methods In vitro cultured human retinoblastoma cells (Hxo-Rb44) were incubated for 24 hours in serum-free RPMI-1640 with 0, 5, 10 and 20 μg/ml of LN. An immunohistochemistry staining SP method and reverse transcriptase -polymerase chain reaction (PT-PCR) were used to detect the effect of LN on the expression of MMP-2, MMP-9 and their mRNA. The differences in the expression of MMP-2, MMP-9 and their mRNA in each experimental group were analyzed statistically. Results The expression of MMP-2, MMP-9 and their mRNA increased significantly after co-culture with LN. Immunohistochemistry results showed that the respective densities of MMP-2 and MMP-9 in 5、10 and 20 μg/ml for the LN treatment groups were 178.1±11.7 and 167.0±15.7、147.9±13.4 and 140.3±12.0、114.4±13.6 and 104.0±10.6, respectively. The differences in the expression of MMP-2 and MMP-9 in the LN treated group increased significantly (P 0.05) compared to the control group, and the higher the concentration of LN, the higher the expression of MMP-2 and MMP-9. The respective density ratios of MMP-2/β-actin and MMP-9/β-actin in 5、1 and 20 μg/ml for the LN treated groups were 0.181±0.016 and 0.254±0.023、0.201±0.021 and 0.287±0.020、and 0.243±0.019 and 0.320±0.022, respectively. The difference in the expression of their mRNA was statistically significant(P 0.05)compared to the control group. There was an obvious concentration-dependent relationship, the higher the concentration of LN, the higher the mRNA expression of MMP-2 and MMP-9. Conclusion It was confirmed that LN increases the expression of MMP-2, MMP-9 and their mRNA in retinoblastoma cells in a concentration-dependent manner, which suggested LN might promote the invasion and metastasis of Rb cells." @default.
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- W2357802605 date "2007-01-01" @default.
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- W2357802605 title "The impact of laminin on the expression of MMP-2 and MMP-9 in human retinoblastoma cells" @default.
- W2357802605 hasPublicationYear "2007" @default.
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