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- W2357813867 abstract "Calcium alginate hydrogels is an appealing scaffold material because it is structurally similar to the extracellular matrix of many tissues,can often be processed under relatively mild conditions and may be delivered in a minimally invasive manner.In this paper,sodium alginate (SA),calcium carbonate and glucono-delta-lactone (GDL) were selected as the materials to prepare the injectable calcium alginate hydrogel using in-situ transformation method. The influences of different concentrations of SA,f value (f=Ca2+/COO-),n value (n=Ca2+/GDL) on the hrdrogel mechanical strength,swelling ratio and pH value of the leaching liquor were investigated and the optium gelation condition was finally determined as following:concentration of SA 2.5%,f=0.5,and n=0.6.In addition,the chondrocytes were seeded homogeneously in the gel by in-situ cell culture and were analyzed by live-dead staining after 1,3 and 5d.Results showed that the cell activity was high and the chondrocytes morphology was well maintained,which overcomed the deficiency that chondrocytes tend to differentiate to fibroblasts at one-dimensional or two-dimensional culture.The results further confirmed that calcium alginate hydrogel is a potential injectable scaffolds materials for cartilage tissue engineering." @default.
- W2357813867 created "2016-06-24" @default.
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- W2357813867 date "2010-01-01" @default.
- W2357813867 modified "2023-09-24" @default.
- W2357813867 title "Preparation and characterization of calcium alginate hydrogels as injectable cartilage scaffolds" @default.
- W2357813867 hasPublicationYear "2010" @default.
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