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- W2357824219 abstract "Objective To observe the effect of PDCD5 protein on the apoptosis of multiple myeloma (MM) KM3 cells induced by dexamethasone. Methods The rhPDCD5 protein were added alone of different concentrations or conjuncted with dexamethasone to KM3 cells in phase of logarithmic growth and cultured for 16 h,then the cells were collected after co-culture for the following experiments. Cell morphology was observed by inverted microscope directly and by fluorescence microscope after staining with DAPI. Effects of rhPDCD5 protein and dexamethasone on the apoptotic rate of KM3 cells were detected by Annexin V-FITC and PI double staining. Results Cell shrinkage,membrane protrusions and irregular bubble-shaped depression,nuclear pyknosis,nuclear fission and other morphological characteristic of apoptosis were observed in PDCD5 combined with dexamethasone group,while in single-treatment groups there were some but relatively little morphological changes of apoptosis. PBS control group had no significant changes in cell morphology. Apoptotic rate increased more obviously in the combination groups than in the single-treatment groups. Conclusions rhPDCD5 protein can enter MM cells KM3,induce apoptosis of KM3 cells and accelerate apoptosis of KM3 cells induced by dexamethasone." @default.
- W2357824219 created "2016-06-24" @default.
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- W2357824219 date "2010-01-01" @default.
- W2357824219 modified "2023-09-23" @default.
- W2357824219 title "Effect of human recombinant PDCD5 protein on cell apoptosis of KM3 multiple myeloma cells induced by dexamethasone" @default.
- W2357824219 hasPublicationYear "2010" @default.
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