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- W2357843596 abstract "Quantum dots have physical and optical properties that make them useful tools for high-resolution labeling immunoassay.In this work,a rapid and simple method of quantitative immunoassay for Cardiac troponin I(cTnI) was developed with quantum dots-labeled antibodies.The monoclonal antibodies of cTnI(2F11) could be labeled with CdTe quantum dots and the coupled product(CdTe-2F11) were characterized by SDS-PAGE.The result of immunofiltration assay indicats that the CdTe-2F11 maintains the antibody activity.The cTnI at the different concentrations in NC membrane could react with CdTe-2F11 and be detected with ImageMaster to analyze the fluorescence intensity of the immunodotting.The results show that the detection limit of cTnI is 120 ng,and there is a good linear relation between concentration of cTnI and the fluorescence intensity(R2=0.9966)." @default.
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- W2357843596 date "2009-11-10" @default.
- W2357843596 modified "2023-09-26" @default.
- W2357843596 title "Dot immunofiltration assay for quantitative detecting cTnI using quantum dots" @default.
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