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- W2357844512 abstract "In this paper,the method for smearing of Betula playphylla callus was tested;and the chromosome variation of callus and the regenerated plantlets from callus were observed.The results indicated that the callus,which were cultured for one week was the best material for observed chromosome.It was easier to obtain ideal division cells by the method that callus were treated with 0.2% colchicine for 1.5～2h.The optimum hydrolyze condition was that the callus was soaked in 1 mol/L HCl for 10～15min or in 2% pectinase and cellulase for 30min at room temperature.Wide-ranging chromosome variation was existed in Betula platyphylla callus,but most variations tended to the decreased of normal chromosomal number.The ratio and range of chromosome variation were increasing along with the length of culture time.The variation rule of chromosome was similar among different genotypes.The variation of chromosome number of regenerated plants from callus was lower than callus." @default.
- W2357844512 created "2016-06-24" @default.
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- W2357844512 date "2011-01-01" @default.
- W2357844512 modified "2023-09-28" @default.
- W2357844512 title "Study of the method for smearing of Betula platyphylla callus and the number variation" @default.
- W2357844512 hasPublicationYear "2011" @default.
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