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- W2357876611 abstract "Polyphenol oxidase (PPO) activity is highly related to the undesirable browning of wheat-based end products. In this study, wheat (Triticum aestivum ) PPO sequences (mRNA) were searched/blasted in NCBI and aligned using DNAMAN software. The results showed that the wheat PPO genes could be divided into two clusters (Ⅰ and Ⅱ) and three genes ( i ) of the Ⅱ cluster did not seem on chromosomes 2A and 2D. Ninety-four single nucleotide polymorphisms (SNP) were detected between two haplotypes of PPO gene on chromosome 2D. Eighty SNPs of them were found in coding region (coding SNP) and 36 SNPs were non-synonymous cSNPs, which could affect the PPO amino acid sequence. Primers was designed as STS-H at some non-synonymous cSNPs sites and used to research the correlations between allelic variants and PPO activity of seeds, which valued in two years in total of 130 common wheat varieties. The results showed that the STS-H could amplify a 460 bp DNA fragment in most cultivars with high PPO activity (b), while no PCR product was detected in most cultivars with low PPO activity (a), and the former cultivars (a) were proven to have significantly (P 0.01) higher mean PPO activity than that of the latter (b). To improve the selection efficiency of single dominance molecular marker, the multiplex PCR system of STS-H and STS01 markers was also studied, based on the complementary between them." @default.
- W2357876611 created "2016-06-24" @default.
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- W2357876611 date "2007-01-01" @default.
- W2357876611 modified "2023-09-23" @default.
- W2357876611 title "Classification of Wheat Polyphenol Oxidase (PPO) Genes and Effect of Non-synonymous cSNP on Kernel PPO Activity" @default.
- W2357876611 hasPublicationYear "2007" @default.
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