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- W2357877899 abstract "Subtracted clone BF475207 had a higher expression in sperm cells than in two cell stage pollen. The inserted fragment of this clone was amplified via PCR method and labeled as a probe to screen the cDNA library of rice sperm cells. One 1 176 bp gene containing an open reading frame of 281 amino acids was cloned and named as RSG 6 (accession number in GenBank: AF442490). It is the first gene isolated directly from sperm cells of higher plants with complete sequence. Sequence of RSG 6 and the deduced amino acid sequence did not reveal remarkable similarity to any known sequences in GenBank, showing that it was a new gene. Reverse transcriptase polymerase chain reaction analysis showed that RSG 6 gene expressed in all rice organs or cell types (including roots, leaves, two cell stage pollen, mature pollen and sperm cells), but the level of expression in sperm cells was particularly higher, which suggested that RSG 6 be a specially abundant expressed gene of rice sperm cells. The open reading frame of RSG 6 gene was cloned into expression vector pQE30. After induction with IPTG, RSG 6 gene was expressed in E.coli M15 (pREP4) consisting of 6×His fused to the N terminal. The purified protein as antigen was used to immunize rabbits and high specific and high titer antibody was obtained." @default.
- W2357877899 created "2016-06-24" @default.
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- W2357877899 date "2002-01-01" @default.
- W2357877899 modified "2023-09-24" @default.
- W2357877899 title "CLONING OF A DIFFERENTIALLY EXPRESSED GENE IN RICE SPERM CELLS AND PREPARATION OF ANTISERUM AGAINST EXPRESSED PRODUCT" @default.
- W2357877899 hasPublicationYear "2002" @default.
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