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- W2357879425 abstract "With the method of single-spore isolation an fstrain of Sclerotinia sclerotiorum was isolated from oilseed rape(Brassica napus)in Zhoukou city of Henan province,The morphology and microscopic analysis indicated that the isolated stain was S.sclerotiorum.Universal PCR primers ITS1and ITS4for fungi were used to amplify the internal transcribed spacer(ITS)of the isolated strain,which resulted in PCR fragment with 540bp.The PCR product was sequenced and analyzed by using BLASTn,which showed 100%homology to ITS sequences of some S.sclerotiorumstains.It further proved that the isolated strain was S.sclerotiorum.Phylogenetic tree analysis demonstrated that the isolated strain of S.sclerotiorum in Zhoukou was close to stains from Italy,Japan and Wuhan of China,suggesting that the origin of S.sclerotiorumin Zhoukou may be diverse." @default.
- W2357879425 created "2016-06-24" @default.
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- W2357879425 date "2013-01-01" @default.
- W2357879425 modified "2023-09-26" @default.
- W2357879425 title "Identification of Sclerotinia sclerotiorumon Oilseed Rape in Zhoukou and Analysis of Its Infection on Plants" @default.
- W2357879425 hasPublicationYear "2013" @default.
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