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- W2357893575 abstract "Aim:To construct pcDNA3.1-hTERT,an eukaryotic recombinant plasmid of human telomerase reverse transcription(hTERT),and evaluate its anti-tumor immunization effects in the mice bearing tumor.Methods:Total RNA was extracted from the tumor tissue and the fragment of hTERT cDNA was amplified by RT-PCR.The amplified fragment was cloned into pGEM-T Easy vector.The target fragment was sub-cloned into eukaryotic expression vector pcDNA3.1.The C57BL/6 mice model with transplantable H22 liver cancer,were randomly allocated into three groups as PBS,pcDNA3.1,and pcDNA3.1-hTERT.The anti-tumor effects were measured.Results and Conclusion: The recombinant plasmid pcDNA3.1-hTERT was constructed successfully.The hTERT fragment in pcDNA3.1-hTERT was 951 bp.It encoded the polypeptide with 317 amino acid residues corresponding to calculated molecular masses of 37 000.The homology in nucleotide acid of the hTERT fragment compared with the hTERT gene reported in GenBank was 98.73%,and the homology in amino acids was 99.68%.The result showed that the growth of tumor was slower and the survival time of pcDNA3.1-hTERT group was obviously longer than those of PBS and pcDNA3.1 groups(both P0.05).The levels of IL-2 and IFN-γ in the mice of pcDNA3.1-hTERT group were significantly higher than those of PBS and pcDNA3.1 groups(all P0.05).The recombinant plasmid pcDNA3.1-hTERT can induce anti-tumor immunization effect in the mice bearing tumor." @default.
- W2357893575 created "2016-06-24" @default.
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- W2357893575 date "2008-01-01" @default.
- W2357893575 modified "2023-09-25" @default.
- W2357893575 title "Construction of pcDNA3.1-hTERT eukaryotic recombinant plasmid and its effects of anti-tumor immunization" @default.
- W2357893575 hasPublicationYear "2008" @default.
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