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- W2357921209 abstract "A gene (GenBank AF096282) coding for a α-glucosidase (TcaAG, EC 3.2.1.20) from Thermus caldophilus GK24 was expressed in Saccharomyces cerevisiae, a generally recognized as safe (GRAS) host. The thermostable α-glucosidase was produced inside of the GRAS host at 0.04 unit/mg-dry cell by the constitutively expressing ADH1 promoter and at 1.2 unit/mg-dry cell by the inductively expressing GAL10 promoter, respectively. No α-glucosidase activities were found in the medium when the MF-alpha signal sequence from S. cerevisiae or α-amylase signal sequence from Aspergillus oryzae were fused before the α-glucosidase gene for the secretion." @default.
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- W2357921209 date "2006-12-01" @default.
- W2357921209 modified "2023-09-28" @default.
- W2357921209 title "Expression of thermostable α-glucosidase from Thermus caldophilus GK24 in recombinant Saccharomyces cerevisiae" @default.
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