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- W2357929524 abstract "OBJECTIVE To observe the effect of theophylline on the shedding of L-selectin from the surface acidophil.METHODS EDTA-anticoagulated venous blood was drawn from 15 healthy volunteers and pre-incubated with theophylline (10 -3、10 -4、10 -5mol·L -1) and rhIL-5(10 -8mol·L -1).The intracellular mRNA of L-selectin was determined by reverse transcriptase polymerase chain reaction (RT-PCR).The intracellular EG 2+ and the L-selectin lipoprotein on surface of acidophil were determined by flowcytometry technique.The relationship between percentage of EG 2+ and level of L-selectin was analysed.RESULTS ①The shedding of L-selectin on IL-5-stimulated acidophil was inhibited by theophylline and dexamethasone.②The theophylline didn't affect on expressing of L-selectin mRNA inside acidophil.③There was a correlation between the percentage of EG 2+ and the level of L-selectin.CONCLUSION Theophylline may affect to the adhesive function of acidophil by inhibiting its activity and its shedding of L-selectin." @default.
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- W2357929524 date "2005-01-01" @default.
- W2357929524 modified "2023-09-25" @default.
- W2357929524 title "The effect of theophylline on the shedding of L-selectin from acidophil" @default.
- W2357929524 hasPublicationYear "2005" @default.
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