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- W2357946947 abstract "To establish a fluorogenetic quantitative RT-PCR assay for detection of H5N1 tiger influenza A virus (TIV), the HA gene of TIV was aligned using the biologic software and the specific primers were designed in the conserved region of the HA gene by Primer 5.0. For SYBR Green I was cheaper and can be used to distinguish variant of virus, the SYBR Premix Ex TaqTM system was selected. The primers and the reactive condition were optimized to improve the sensitivity and specificity of the assay. The sensitivity of the assay was 10~1~10~2 copies. Twenty clinical samples from died tigers and 24 samples of mice infected with A/Tiger/Harbin/01/2003 (H5N1) were detected by the fluorogenetic quantitation RT-PCR, routine RT-PCR and virus isolation,recpectively. The sensitivity of the method of fluorogenetic quantitation RT-PCR was higher than routine RT-PCR, but similar with virus isolation. The result indicated that fluorogenetic quantitation RT-PCR may be used as a good reference in detection of H5N1 TIV." @default.
- W2357946947 created "2016-06-24" @default.
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- W2357946947 date "2006-01-01" @default.
- W2357946947 modified "2023-09-25" @default.
- W2357946947 title "Development and clinical application of fluorogenetic quantitativeRT-PCR for detection of H5N1 tiger influenza A virus" @default.
- W2357946947 hasPublicationYear "2006" @default.
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