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- W2357993118 abstract "Objective To establish nest-PCR method of Brucella .Methods According to B.abortus 31KD gene,a set of primers to detect Brucella were designed.The Production of the PCR assay is 223 bp.The optimal condition of PCR for detection of Brucella was determined through orthogonal assay.The specificity of the sets of primers were examined by PCR using template extracted from of 6 species Brucella,Y.enterocolitica O:9,E.coli 157 and S.tyPhimuium strains.While the sensitivity of PCR was also determined by a seral dilution of Brucella. Results The DNA of 6 species Brucella strains were amplified by the primers of 31 KDa,but not for the DNA of Y.enterocolitica O:9,E.coli 157 and S.typhimuium strains,about 20 CFU can be detected.This shows that the nest-PCR method of Brucella is specificity and sensitivity.Conclusions Nest-PCR method of Brucella has been established in this study,and veritified to be specificity and sensitivity.The study has paved the way for testing of Brucella." @default.
- W2357993118 created "2016-06-24" @default.
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- W2357993118 date "2006-01-01" @default.
- W2357993118 modified "2023-09-24" @default.
- W2357993118 title "Establishment of Rapid Test of Brucella by Nest-PCR" @default.
- W2357993118 hasPublicationYear "2006" @default.
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