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- W2358007948 abstract "[Objective] The research aimed to purify and analyze the exopolysaccharides(EPS) from Lactobacillus casei.[Method] The EPS synthesized by Lactobacillus casei LC2W were fractionated by anion exchange chromatography on DEAE-Sepharose Fast Flow,then gel filtration chromatography on Sepharose CL-6B column and HPLC were used to further analyze the single component.[Result] Three peaks G1,G2 and G3 were obtained by separating and purifying the EPS from Lactobacillus casei,accounted for 21.33%,33.53%,29.67% of the EPS obtained.The rate of EPS recovery was 84.53%,G1 and G2 were all neutral polysaccharides.The results of gel filtration chromatography on Sepharose CL-6B column and HPLC showed that G1 and G2 fractions were single components,and the average weight molecular weight(Mw) of G1 and G2 were 6.65×105,1.01×104 Da respectively.[Conclusion] The study can provide the reference for deeply studying the structure and biological activity of EPS from Lactobacillus casei." @default.
- W2358007948 created "2016-06-24" @default.
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- W2358007948 date "2010-01-01" @default.
- W2358007948 modified "2023-09-22" @default.
- W2358007948 title "Study on Purification and Analysis of Exopolysaccharides from Lactobacillus casei" @default.
- W2358007948 hasPublicationYear "2010" @default.
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