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- W2358056474 abstract "Objective To establish the human placental trophoblast(HPT-8) cells for replicating HBV by transfection of pcDNA3-3HBV adr subtype in vitro.Methods HPT-8 cells were transfected with eukaryotic expression plasmid pcDNA3-3HBV by using lipofectamine transfection reagent,after being screened with antibiotic G418.HBsAg and HBeAg were identified by enzyme linked immunosorbent assay(ELISA),and HBV DNA was tested by polymerase chain reaction (PCR) in the supernatant of transfected HPT-8 cells.HBcAg and HBsAg were identified by immunocytochemical staining in the transfected HPT-8 cells.Results After HPT-8 cells were stably transfected with pcDNA3-3HBV,the ELISA showed,.in the supernatant of transfected HPT-8 cells at passage1 and passage2,HBsAg and HBeAg were positive;the immunocytochemistry showed,in the plasma of transfected HPT-8 cells,HBsAg and HBcAg were of positive staining;the PCR revealed positive HBV DNA gene in the supernatant;the real-time quantitative PCR showed copies of HBV genome reach 9×10~6 copies /L,and RcDNA and cccDNA was found in the HPT-8-HBV cells.Conclusion Recombinant plasmid pcDNA3-3HBV could be expressed,transcribed and replicated in HPT-8 cells." @default.
- W2358056474 created "2016-06-24" @default.
- W2358056474 creator A5071368242 @default.
- W2358056474 date "2008-01-01" @default.
- W2358056474 modified "2023-09-24" @default.
- W2358056474 title "Human Placental Trophoblast Cell Strain Transfected with Hepatitis B Virus Adr Subtype In Vitro" @default.
- W2358056474 hasPublicationYear "2008" @default.
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