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- W2358076384 abstract "Objective:To construct the DNA vaccine against herpes simplex virus type Ⅰ glycoprotein B(HSV-Ⅰ,gB) and evaluate its effects on cell-mediated immunity.Methods:The gB encoding sequence(2 673 bp) with the removal of signal peptide(39 bp) was amplified by polymerase chain reaction(PCR),and then was directionally cloned into the vector pcDNA3,the recombinant vector pcDNA3-gB was confirmed by the restricted endonuclease analysis,PCR and sequence analysis.It was employed to evaluate immune response of the mice inoculated triply with the DNA vaccine.The transformation of CD4+/CD8+T lymphocyte was detected and the influence of cell-mediated immunity represented by CTL was assayed through CFSE/PI flow cytometry.Results:Double-enzyme digestion showed two bands which are HSV-Ⅰ gB gene(2.7 kb) and linear pcDNA3(5.4 kb);PCR analysis shows the specific band(2.7 kb);sequence analysis showed the rate of homology was 99.5% compared with GenBank.The mice immunized with pcDNA3-gB induced high levels of CTL;the amount of CD4+T cell increased.Conclusion:The construction of the HSV-Ⅰ DNA vaccine is identified;it is powerful to induce cell-mediated immunity and is promising for the treatment of HSV-Ⅰ infection." @default.
- W2358076384 created "2016-06-24" @default.
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- W2358076384 date "2005-01-01" @default.
- W2358076384 modified "2023-09-25" @default.
- W2358076384 title "The construction and cellular immunological study of herpes simplex virus type I glycoprotein B DNA vaccine" @default.
- W2358076384 hasPublicationYear "2005" @default.
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