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- W2358077698 abstract "A real-time quantitative RT-PCR assay was developed for detection of Norwalk-like Virus(NLV) from oysters.Primers and probes were designed based on the RNA polymerase region of the virus.The Taqman probe was labeled with fluorescent dye FAM on the 5' end and TAMRA on the 3' end.The real-time RT-PCR assay had a detection limit of 6 virus copies,with a dynamic range of detection between 6×106 to 6 virus copies.The standard curve was prepared based on the linear relationship between the amount of plasmid DNA(X) and cycle threshold(Ct).For NLV virus,Ct =-3.36 lgX+ 37.11,with the correlation coefficient R2=0.998.Thirty-seven oysters were tested and six were NLV positive and the virus was quantified.The results demonstrate that real-time RT-PCR method can be used as a rapid and highly sensitive detection and quantification method for NLV and it is amenable to high-throughout assay." @default.
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- W2358077698 date "2008-01-01" @default.
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- W2358077698 title "Detection of Norwalk-like virus in oysters(Crassostrea gigas) by real-time quantitative RT-PCR" @default.
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