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- W2358085667 abstract "Objective To deect the inhibition of RNAi techinque to the expression of E2F1gene in breast cancer cell line MCF-7 and investigate the expression of TS gene. Method Recombinant plasmid pSIREN-E2F1 was constructed and transfected into human breast cancer cell line MCF-7. And then selected the stable transfected cells scaling up culture. The expression of E2F1 and TS gene were detected by RT-PCR. Result The expression of E2F1mRNA and TSmRNA were suppressed in pSIREN-E2F1 transfected cells.Conclusion RNAi can effectively suppress the expression of E2F1 and TS gene in human breast cancer cell and provide an effective anti-cancer gene therapy." @default.
- W2358085667 created "2016-06-24" @default.
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- W2358085667 date "2008-01-01" @default.
- W2358085667 modified "2023-09-23" @default.
- W2358085667 title "Inhibition effect of RNAi to E2F1 and TS genes expression in human breast cancer MCF-7 cell" @default.
- W2358085667 hasPublicationYear "2008" @default.
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