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- W2358094836 abstract "Objective To study the effects of different concentrations of human amniotic membrane homogenate extraction on proliferation of cultured corneal fibroblasts and the expression of transforming growth factor-β1(TGF-β1) mRNA in rabbits,and investigate the mechanism of human amniotic membrane homogenate extraction inhibiting the corneal scar.Methods The primary culture of the rabbit corneal fibroblasts gained by digestion and serial subcultivation,identified with vimentin and keratin immunocytochemistry.The effects of different concentrations(0 mg·L-1,40 mg·L-1,80 mg·L-1,160 mg·L-1) human amniotic membrane homogenate extraction on proliferation of corneal fibroblasts by MTT colorimetric assay,and the expression of TGF-β1 mRNA in cultured corneal fibroblasts of rabbit was detected by RT-PCR.Results Positive reaction of cellular vimentin and negative reaction of cellular keratin in cultured fibroblasts of rabbit cornea confirmed that the cultured cells were fibroblasts of rabbit cornea by immunocytochemistry.OD values gained by MTT were significant different among the experiment groups(0.335±0.013,0.220±0.018,0.094±0.005) and control ones(0.415±0.026)(all P0.01),which showed the proliferation of corneal fibroblasts was inhibited by different concentrations of human amniotic membrane homogenate extraction.The ralative expression of TGF-β1 mRNA was more significantly depressed in different concentrations of experimental groups than in control ones,there were significant differences among different concentrations of experimental groups(1.519±0.669,0.617±0.014,0.622 ±0.031) and control ones(1.768 ±0.040)(all P0.01).But there was no significant difference between the midrangeconcentrations and high ones(80 mg·L-1 and 160 mg·L-1)(P0.05).Conclusion The human amniotic membrane homogenate extraction significantly inhibits the proliferation of cultured corneal fibroblasts in rabbit in vitro.The inhibitive effects increase with the increasing concentrations of human amniotic membrane homogenate extraction." @default.
- W2358094836 created "2016-06-24" @default.
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- W2358094836 date "2013-01-01" @default.
- W2358094836 modified "2023-09-28" @default.
- W2358094836 title "Effects of of human amniotic membrane homogenate extraction on proliferation of corneal fibroblasts and expression of TGF-β1 mRNA in rabbit" @default.
- W2358094836 hasPublicationYear "2013" @default.
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