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- W2358099586 abstract "In order to analysis variation of rabbit hemorrhagic disease virus isolate,and obtain good active of gene engineering antigens,the research conducted the sequence analysis and prokaryotic expression of rabbit hemorrhagic disease virus ZB strain VP60 gene.The isolated ZB viruses compared with 6 strains of RHDV VP60 gene in GenBank,the homology of the nucleotide sequence is 92.5% to 97.9%.According to ZB strain VP60 gene sequence,one pairs of primers were designed,876 bp fragment of VP60 gene was amplified by PCR.The fragment was directionally cloned into pET30 aexpression vector.Aafter identification,the recombinant plasmid was transformed into BL21 expression bacteria and induced by IPTG,the recombinant protein was expressed in inclusion bodys forms,the recombinant protein was purified.Western blot detection showed that the expressed protein had good antigenicity and specificity." @default.
- W2358099586 created "2016-06-24" @default.
- W2358099586 creator A5063008071 @default.
- W2358099586 date "2013-01-01" @default.
- W2358099586 modified "2023-09-25" @default.
- W2358099586 title "Cloning,Truncated Expression and Activity Identification of Rabbit Hemorrhagic Disease Virus VP60 Gene of ZB Strain" @default.
- W2358099586 hasPublicationYear "2013" @default.
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