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- W2358133604 abstract "In this study, five FMDV type O epidemic strains were gathered.RT-PCR was employed to amplify the cDNAs of the 3ABC gene of five strains with a pair of specific oligonucleotide primers (3A/3C).The cDNAs were cloned into the T-T windows of plasmid pMD18-T vector respectively.The inserts were sequenced and the results showed that the full-length sequences of the 3ABC gene of all the five strains consisted of 1281 nucleotides (nt) respectively,coding for predicated polypeptides of 427 amino acid residues.Analyses of the 3ABC gene nucleotide sequences and deduced amino acid sequences suggested that five FMDV type O epidemic strains shared (100?%) identity.Comparison of the homologies of the 3ABC genes showed that there was 96.8?%～98.4?% nucleotide sequences homology and 97.2?%～98.8?% deduced amino acid sequence homology among the five strains.Based on the results of nucleotide sequence and deduced amino acid sequence homologies,the phylogenetic trees of the 3ABC gene were constructed.The positions of the five strains were highly related between the two trees." @default.
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- W2358133604 date "2005-01-01" @default.
- W2358133604 modified "2023-09-24" @default.
- W2358133604 title "Cloning and sequencing of FMDV Type O 3ABC gene" @default.
- W2358133604 hasPublicationYear "2005" @default.
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