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- W2358156798 abstract "Objective To establish the molecular weight distribution of Ganlong capsule by HPSEC the content of the peptide determined by Lowry and Methods The superdex peptide 10 /300 GL(10 mm × 300 mm) column was used.The p H = 6.0 and phosphate buffer of 0.05 mol / L was used as the mobile phase,containing 0.1 mol / L Na Cl.The flow rate was set at 0.7 m L / min; The column temperature was 25 ℃; The detection wavelength was 214 nm.Results The content of the peptide ranged from 0.08 mg to 0.4 mg(r = 0.9996).The RSDs of measurement precision of molecular weight and content were 0.08% and 0%(n = 6),respectively.The RSDs of the repeatability were 1.3% and 1.1%(n = 6); The regression equation of standard material was log Mr = 5.1455-0.0871 tR,r = 0.9983,the relative molecular weight ranged from 2.68 × 102 Da ~ 5.73 × 103Da(r = 0.9983).Conclusion on The method is simple and rapid for determining the peptide content and the molecular weight distribution of Ganlong capsule.It can be used quality control method for Ganlong capsule." @default.
- W2358156798 created "2016-06-24" @default.
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- W2358156798 date "2015-01-01" @default.
- W2358156798 modified "2023-09-26" @default.
- W2358156798 title "Research on the determination of molecular weight distribution and the peptide content of Ganlong capsule" @default.
- W2358156798 hasPublicationYear "2015" @default.
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