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- W2358164524 abstract "The pieces of sterile seeding of Astragalus membranaceus Bunge Var.mengholicus(Bunge) Hsiao were infected with [WTBX]Agrobacterium rhizogenes strains A_4 and R_(1601).The transformants(hairy roots and transformed calluses) were obtained by selection on hormone-free medium.The transformed root cutures were assayed by TLC,with positive result for opines.The effect of several factors on transformation frequency was studied.The results indicated that Strain R_(1601) has higher transformation frequency than that of A4,and the explant hypocotyls has the hightest.MS medium,three days preculture and four days prcoculture were found as the best transformation conditions with the addition of acetosyringone(100μg/L) and IBA(0.3 mg/L),and the rate of inducing hairy roots was 42.2 percent." @default.
- W2358164524 created "2016-06-24" @default.
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- W2358164524 date "2006-01-01" @default.
- W2358164524 modified "2023-09-25" @default.
- W2358164524 title "Studies on factors affecting the transformation of Astragalus mombranaceus Bunge Var.mongholicus with Agrobacterium rhizogenes" @default.
- W2358164524 hasPublicationYear "2006" @default.
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