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- W2358217353 abstract "Objective To obtain the rabbit-anti-human matrix metalloproteinase-11(MMP-11) polyclonal antibody and establish a new immunological method for detecting menstrual blood quickly and accurately in forensic field.Methods The fusion protein,obtained by genetic engineering technique,was used to immunize female rabbits,and antibodies obtained from the immune sera were purified by ammonium sulfate fractionation,their specificity and capability of distinguishing menstrual blood from peripheral blood,vaginal liquid,salivary stain,semen,urine and other biological marks were assayed with Western blot methods.Results The purified specific polyclonal antibodies of rabbit-anti-human MMP-11 were obtained.Tissue specificity of antibodies was evident in menstrual blood by Western blot detection,compared with venous blood,vaginal fluid and other body fluids.Conclusion Western blot analysis with MMP-11 polyclonal antibody can be applied to detect menstrual blood in forensic examination." @default.
- W2358217353 created "2016-06-24" @default.
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- W2358217353 date "2007-01-01" @default.
- W2358217353 modified "2023-09-23" @default.
- W2358217353 title "Preparation of anti-human MMP-11 polyclonal antibody for forensic application" @default.
- W2358217353 hasPublicationYear "2007" @default.
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