Matches in SemOpenAlex for { <https://semopenalex.org/work/W2358311021> ?p ?o ?g. }
Showing items 1 to 71 of
71
with 100 items per page.
- W2358311021 abstract "Objective To clonethe ORF of murine B cell activating factor of TNF family(BAFF),express in prokaryotic cells and purify the expressed product.Methods Total RNA was extracted from fresh spleen of mice,from which the ORF of BAFF was amplified by RT-PCR and cloned into vector pMAL-c2x.The constructed recombinant plasmid was transformed to E.coli Rosseta(DE3)for expression under induction of IPTG.The expressed product was purified by affinity chromatography and analyzed by Western blot.Results A cDNA fragment at length of about 930 bp was amplified by RT-PCR,of which the homology was 99.9% to the ORF cDNA sequence reported in GenBank.Restriction analysis proved that recombinant plasmid pMAL-c2x /BAFF ORF was constructed correctly.The relative molecular mass of expressed recombinant protein was about 76 500.The expression level reached a peak value after induction with IPTG at a final concentration of 0.9 mmol/L for 4 h.Target protein bands were observed in both supernatant and precipitate of ultrasonicated E.coli.The expressed product in soluble form contained 49% of total somatic protein.The purified recombinant protein reached a purity of 90% and a content of 0.9 mg/ml,and showed good reactogenicity.Conclusion Murine BAFF ORF was successfully cloned,expressed in prokaryotic cells and purified,which laid a foundation of further study on its biological function." @default.
- W2358311021 created "2016-06-24" @default.
- W2358311021 creator A5086019485 @default.
- W2358311021 date "2010-01-01" @default.
- W2358311021 modified "2023-09-24" @default.
- W2358311021 title "Cloning and Prokaryotic Expression of ORF of Murine B Cell Activating Factor of TNF Family" @default.
- W2358311021 hasPublicationYear "2010" @default.
- W2358311021 type Work @default.
- W2358311021 sameAs 2358311021 @default.
- W2358311021 citedByCount "0" @default.
- W2358311021 crossrefType "journal-article" @default.
- W2358311021 hasAuthorship W2358311021A5086019485 @default.
- W2358311021 hasConcept C104317684 @default.
- W2358311021 hasConcept C121050878 @default.
- W2358311021 hasConcept C153911025 @default.
- W2358311021 hasConcept C159654299 @default.
- W2358311021 hasConcept C170286685 @default.
- W2358311021 hasConcept C170344550 @default.
- W2358311021 hasConcept C187882448 @default.
- W2358311021 hasConcept C199360897 @default.
- W2358311021 hasConcept C22744801 @default.
- W2358311021 hasConcept C2778453870 @default.
- W2358311021 hasConcept C33161422 @default.
- W2358311021 hasConcept C40767141 @default.
- W2358311021 hasConcept C41008148 @default.
- W2358311021 hasConcept C54355233 @default.
- W2358311021 hasConcept C55493867 @default.
- W2358311021 hasConcept C86803240 @default.
- W2358311021 hasConceptScore W2358311021C104317684 @default.
- W2358311021 hasConceptScore W2358311021C121050878 @default.
- W2358311021 hasConceptScore W2358311021C153911025 @default.
- W2358311021 hasConceptScore W2358311021C159654299 @default.
- W2358311021 hasConceptScore W2358311021C170286685 @default.
- W2358311021 hasConceptScore W2358311021C170344550 @default.
- W2358311021 hasConceptScore W2358311021C187882448 @default.
- W2358311021 hasConceptScore W2358311021C199360897 @default.
- W2358311021 hasConceptScore W2358311021C22744801 @default.
- W2358311021 hasConceptScore W2358311021C2778453870 @default.
- W2358311021 hasConceptScore W2358311021C33161422 @default.
- W2358311021 hasConceptScore W2358311021C40767141 @default.
- W2358311021 hasConceptScore W2358311021C41008148 @default.
- W2358311021 hasConceptScore W2358311021C54355233 @default.
- W2358311021 hasConceptScore W2358311021C55493867 @default.
- W2358311021 hasConceptScore W2358311021C86803240 @default.
- W2358311021 hasLocation W23583110211 @default.
- W2358311021 hasOpenAccess W2358311021 @default.
- W2358311021 hasPrimaryLocation W23583110211 @default.
- W2358311021 hasRelatedWork W2178538623 @default.
- W2358311021 hasRelatedWork W2350291426 @default.
- W2358311021 hasRelatedWork W2354108814 @default.
- W2358311021 hasRelatedWork W2357168565 @default.
- W2358311021 hasRelatedWork W2357307206 @default.
- W2358311021 hasRelatedWork W2358562707 @default.
- W2358311021 hasRelatedWork W2360459236 @default.
- W2358311021 hasRelatedWork W2364742102 @default.
- W2358311021 hasRelatedWork W2367199444 @default.
- W2358311021 hasRelatedWork W2370596738 @default.
- W2358311021 hasRelatedWork W2373125649 @default.
- W2358311021 hasRelatedWork W2375474188 @default.
- W2358311021 hasRelatedWork W2377836892 @default.
- W2358311021 hasRelatedWork W2391039623 @default.
- W2358311021 hasRelatedWork W2392042311 @default.
- W2358311021 hasRelatedWork W2396957173 @default.
- W2358311021 hasRelatedWork W2403487157 @default.
- W2358311021 hasRelatedWork W2419467415 @default.
- W2358311021 hasRelatedWork W2423933461 @default.
- W2358311021 hasRelatedWork W3146252746 @default.
- W2358311021 isParatext "false" @default.
- W2358311021 isRetracted "false" @default.
- W2358311021 magId "2358311021" @default.
- W2358311021 workType "article" @default.