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- W2358347304 abstract "Objective To optimize the experimental conditions for single cell gel electrophore sis assay (SCGE). Methods Orthogonal experiment (4 factors×3 levels ) were carried out: unwinding time,gel layer number,electrophoresis time,and electrophoresis voltage. Results Unwinding time was the main factor, followed by the gel layer number,electrophoresis time,and electrophoresis vol tage.Furthermore,number one level under four factors was optimum level. Conclusion Optimized experimental conditions consisted of 20-minute unwinding time,gel of 3?layers,20-minute electrophoresis time,and 20?volt electrophoresis v oltages.." @default.
- W2358347304 created "2016-06-24" @default.
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- W2358347304 date "2004-01-01" @default.
- W2358347304 modified "2023-09-22" @default.
- W2358347304 title "Orthogonal experiment of single cell gel electrophoresis assay using minimal peripheral blood samples" @default.
- W2358347304 hasPublicationYear "2004" @default.
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