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- W2358395989 abstract "OBJECTIVE To develop a high performance liquid chromatography(HPLC)method to determine the content of ATP,ADP and AMP in myocardium of rats,and to observe the change of adenosine phosphates in chronic heart failure which resulted from the chronic excessive post-load.METHODS Male SD rats were divided into abdominal aortic-banded or sham-operated groups.The model of rats with pressure overload-induced chronic heart failure were established by partially banding abdominal aorta.HPLC analysis was preformed using a VP-ODS C18 column(250 mm×4.6 mm,5 μm).The mobile phase consisted of 50 mmol·L-1 tribasic potassium phosphate buffer solution(pH 6.5).The flow-rate was 1 mL·min-1 and the temperature of column was about 25 ℃.The detection wavelength was 259 nm and the injection volume was 20 μL.RESULTS The calibration curve showed good linearity(r=0.999 7-0.999 9)and the average recovery rates of ATP,ADP and AMP were 96.30%,97.64% and 103.28%.The within-day and between-day precisions were less than 1.15% and 4.13%,respectively.The study showed that as compared with sham-operated groups,ATP and ADP levels were significantly decreased in abdominal aortic-banded group,but AMP levels were increased significantly.CONCLUSION This analytical method is simple,rapid and specific in detecting the content of adenosine phosphates in myocardium of rats." @default.
- W2358395989 created "2016-06-24" @default.
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- W2358395989 date "2009-01-01" @default.
- W2358395989 modified "2023-09-24" @default.
- W2358395989 title "Determination of adenosine phosphates in myocardium of rats with pressure overload-induced chronic heart failure by HPLC" @default.
- W2358395989 hasPublicationYear "2009" @default.
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