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- W2358469993 abstract "Objective: To establish a simple, fast and effective DNA extraction method from transgenic rice. Methods: DNA extraction methods including traditional CTAB method, improved alkali-treated method and boiling-treated method for DNA extraction from transgenic brown rice, non-transgenic brown rice and transgenic milled rice were compared to screen the optimal extraction method. Results: The integrity, purity and PCR result of template DNA extracted by improved alkali-treated method were similar with the DNA extracted by traditional CTAB method. Meanwhile, compared with traditional CTAB method, improved alkali-treated method had higher DNA extraction rate, less consuming time and lower cost. Conclusion: Improved alkalitreated method can replace traditional CTAB method as DNA extraction method for the detection of transgenic rice." @default.
- W2358469993 created "2016-06-24" @default.
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- W2358469993 date "2010-01-01" @default.
- W2358469993 modified "2023-09-23" @default.
- W2358469993 title "Comparison of DNA Extraction Methods for Transgenic Rice" @default.
- W2358469993 hasPublicationYear "2010" @default.
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