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- W2358476068 abstract "Porphyra haitanensis is the most economically important seaweeds in south China.The factors which affect the ISSR analysis in the study of the genetic diversity and variety identification of P.haitanensis,such as the amount of template DNA,Taq DNA polymerase,primers concentration,Mg2+ concentration,dNTP concentration and annealing temperature,were studied for optimizing conditions of the ISSR-PCR.The results showed that the optimal conditions being suitable for ISSR-PCR of P.haitanensis were as follows: 25 μL reaction system containing 2.5 μL 10×PCR Buffer,5 ng template DNA,2.5 mmol/L Mg2+,1.5 U Taq DNA polymerase(produced by Takara company),200 nmol/L primers and 200 μmol/L dNTP.With an MJ thermal cycle optimal amplification program was 1 cycle of 7 min at 94℃;35 cycles of 1 min at 94℃,45 s at 48℃,and 2 min at 72℃;1 cycle 7 min at 72℃,using block control style.All these results provided a standardizing ISSR-PCR program for the analysis of genetic diversity and variety identification of P.haitanensis." @default.
- W2358476068 created "2016-06-24" @default.
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- W2358476068 date "2008-01-01" @default.
- W2358476068 modified "2023-09-24" @default.
- W2358476068 title "Establishment and optimization of ISSR reaction system for Porphyra haitanensis" @default.
- W2358476068 hasPublicationYear "2008" @default.
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