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- W2358485860 abstract "Effects of hormones including NAA, IAA, 6-BA on leaf differentiation of Populus euphratica and antibiotics including Kanamycin (Kan), G418, Carbenicillin (Cab), Cefotaxime (Cef) on the growth and differentiation or rooting of various explants of P. euphratica were studied. Genetic transformation system mediated by Agrobacterium and optimum antibiotic concentrations for modified organism selection of P. euphratica were founded. Results shown that: the optimal culture medium for leaf differentiation of P. euphratica was MS+BA 0.5mg/L+NAA 0.1~0.2mg/L+sugar 25g/L+agar 5g/L; at co-culture stage which leaves were used as explants, the optimum concentrations of Kan, G418, Cab and Cef were 10mg/L, 7.5mg/L, 200~600mg/L, 200~400mg/L respectively; at the stage of subculture or rooting of resistance buds, the optimum concentrations were 15~20mg/L, 10~15mg/L, 200~800mg/L, 200~600mg/L respectively." @default.
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- W2358485860 date "2007-07-01" @default.
- W2358485860 modified "2023-09-22" @default.
- W2358485860 title "Establishment of Genetic Transformation System of Populus euphratica and Optimization of Antibiotic Concentration" @default.
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