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- W2358492084 abstract "A pair of primers was designed according to the sequence of LMO1847 of Listeria monocytogenes strain EGD from GenBank.The gene fragment of LMO1847 without signal peptide sequence was obtained by PCR from genomic DNA of L.monocytogenes,and cloned into prokaryotic expression plasmid pET-30a.The recombinant plasmid was transferred into E.coli BL21 and expressed successfully.The purified recombinant protein was used for polyclonal antibody production in rabbits.ELISA analysis showed that the polyclonal antibody of LMO1847 was reactive to whole listeial cells and the polyclonal antibody against the whole cells of L.monocytogenes was reactive to the recombinant LMO1847 protein.Dot-ELISA and the whole bacteria coated ELISA showed that LMO1847 existed on listerial cell surface with good immune reactivity.The expression level of LMO1847 remained stable under short period(3 h)of elevated temperature at 45 ℃,5.5% NaCl or acidic(pH 5.5)stress conditions,but the reaction disappeared under acidic stress at pH 3.0.Expression level of LMO1847 by L.monocytogenes in these conditions was decreased depending on the concentration of salt and temperature variation,and the reaction ability wais steady except grown in 4 ℃." @default.
- W2358492084 created "2016-06-24" @default.
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- W2358492084 date "2007-01-01" @default.
- W2358492084 modified "2023-09-23" @default.
- W2358492084 title "Expression of outer membrane protein LMO1847 of Listeria monocytogenes under stress conditions" @default.
- W2358492084 hasPublicationYear "2007" @default.
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