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- W2358513650 abstract "[Objective] To investigate the effect of Bcl-2 antisense oligodeoxynucleotides(AODN) on the apoptosis of human glioma cell lines U251 induced by cycloheximide(CHX).[Methods] Morphological changes of U251 cells treated with Bcl-2 antisense oligodeoxynucleotides and CHX alone or combined were detected by light microscope.MTT assay were used to investigate the inhibition ratio of different groups.Meantime,apoptosis was identified by DNA electrophoresis.[Results] Bcl-2 antisense oligodeoxynucleotides could obviously enhances the apoptosis of U251 cells indeced by CHX.The apoptosis rates of CHX group,AODN,SODN+CHX and AODN+CHX were higher than those of control group and SODN group(P0.01).The differences among AODN group,SODN+CHX group and AODN+CHX group had statistical significance(P0.01).Bcl-2 ASON make U251 cells more sensitive to CHX.DNA ladder was viewed on agarose gel.[Conclusion] Both Bcl-2 AODN and CHX can enhance the apoptosis of U251 cells.Bcl-2 AODN can obviously enhance the apoptosis of U251 cell induced by CHX." @default.
- W2358513650 created "2016-06-24" @default.
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- W2358513650 date "2007-01-01" @default.
- W2358513650 modified "2023-09-24" @default.
- W2358513650 title "Effect of Bcl-2 antisense oligodeoxynucleotides apoptosis of U251 glioma cells induced by cycloheximide" @default.
- W2358513650 hasPublicationYear "2007" @default.
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