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- W2358532056 abstract "Grain hardness is one of the main characters that determine the processing properties of wheat.Puroindolines,including puroindoline a(Pina) and puroindoline b(Pinb) are major genes controlling grain hardness in wheat.According to the reported conserved regions of Pinb gene,one pair of specific primers were designed and used to amplify Pinb from young leaves genomic DNA and developing endosperm RNA of Aegilops longissima(SS).Through gene cloning and sequencing,one new allele of Pinb was characterized.It was 360bp in size,encoding 119 amino acid residues which corresponded to the structure of mature Puroindoline B(PinB) protein.It contained the tryptophan motif of WPTKWWK and five disulphide bonds formed by ten cysteines,which were specific to Pinb gene in cereal crop.Compared with Pinb-D1a from T.aestivum cv.Capitole,the Pinb genes in Ae.longissima shared 93.3% homologies in nucleotide sequence and 92.4% homologies in amino acid sequence respectively.The expression of Pinb in grain endosperm was verified by RT-PCR.The results showed that Ae.longissima contained grain hardness-controlling gene quite different from that of T.aestivum.It's possible that Aegilops provides an extensive reservoir of genetic resources for cultivated wheat quality improvement by wide crossing." @default.
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- W2358532056 date "2005-01-01" @default.
- W2358532056 modified "2023-09-24" @default.
- W2358532056 title "Cloning and Expression Analysis of Puroindoline b Gene in Aegilops longissima" @default.
- W2358532056 hasPublicationYear "2005" @default.
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