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- W2358570766 abstract "A rapid and simple method based on chip capillary system equipped with a laser-induced fluorescence detector was developed and applied to simultaneous analysis of the multiplex polymerase chain reaction(PCR) products of salmonella and Listeria monocytogenes in food samples.Two pairs of oligonucleotide primers were designed to exclusively amplify invA gene of Salmonella and hly gene of Listeria monocytogenes.The parameters of the multiplex PCR reaction and the chip capillary electrophoresis were optimized.The PCR products were separated and detected by the chip capillary system.The proposed method was employed to simultaneously detect the multiplex PCR products of two foodborne pathogenic bacteria under the optimal conditions within 6 min.The within-day relative standard deviations(RSD)of migration time was in the range of 0.20%~1.7% and the inter-day RSD were from 3.7%~4.5%." @default.
- W2358570766 created "2016-06-24" @default.
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- W2358570766 date "2012-01-01" @default.
- W2358570766 modified "2023-09-24" @default.
- W2358570766 title "Rapid determination of salmonella and listeria monocytogenes in food samples by multiplex PCR-chip capillary electrophoresis" @default.
- W2358570766 hasPublicationYear "2012" @default.
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