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- W2358633721 abstract "Objective To construct a secretable eukaryotic expressing plasmid pcDNA3/sVP1. The immune effects were evaluated by determining the specific neutralizing antibody titration and observing the survival of the mice after challenge with a lethal dose of the virus. Methods The signal peptide DNA sequence of hIL 2 was fused to 5′ terminal of VP1 gene by overlap extension and the sVP1 was cloned into eukaryotic expressing vector pcDNA3 to construct pcDNA3/sVP1. The inserted gene fragment was identified by sequencing and restriction enzyme analysis. BALB/c mice were inoculated with plasmids intramuscularly (i.m.) at 2 week intervals. Thirteen days after every injection, sera were collected and the titer of CVB3 specific neutralizing antibodies was measured. Two weeks after the third immunization, mice were subjected to intraperitoneal (i.p.) challenge with 1000 TCID 50 CVB3 and the numbers of surviving animals were monitored up to 3 weeks post infection. Results The secretable eukaryotic expressing plasmid pcDNA3/sVP1 was successfully constructed. The mean neutralizing antibody titer after the third immunization induced by pcDNA3/sVP1 was much higher than that induced by pcDNA3/VP1( P 0.001 ), showing that pcDNA3/sVP1 could elicit significantly higher level of neutralizing antibody response as compared with pcDNA3/VP1. Conclusion Fusing the signal peptide sequence of hIL 2 to VP1 gene enhanced the neutralizing antibody response of the DNA vaccine." @default.
- W2358633721 created "2016-06-24" @default.
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- W2358633721 date "2004-01-01" @default.
- W2358633721 modified "2023-09-25" @default.
- W2358633721 title "Signal peptide DNA of human IL-2 enhanced neutralizing antibody response of Coxsackievirus B3 VP1 DNA vaccine" @default.
- W2358633721 hasPublicationYear "2004" @default.
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