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- W2358644310 abstract "Objective:To get L-lysine-overproducing mutants and optimal fermentation medium.Method: L-lysine-overproducing mutants were derived from Brebvibacterium flavum XQ-89,by means of mutations with diethyl sulfate(DES) and N-methyl-N′-nitro-N-nitrosoguanidine(NTG),acetic acid and ethanol were added in the fermentation medium,tween-80 and DMSO were added in the fermentation the process.Result: A new L-lysine-overproducing mutant XQ-89(SGrVal-) was obtained and the L-lysine production was accumulated 77g/L under suitable conditions.Using the response surface method(RSM) to optimize the concentrations of acetic acid,Tween-80 and corn steep liquor.As a result,in the optimized media when the concentrations of acetic acid,tween-80 and the corn steep liquor were 0.32%,0.66%,1.5% respectively,L-Lysine obtained was 94g/L,which was over 22.1% higher than that obtained in the basal medium.Conclusion: Screening(SGrVal-) were conducived to the accumulation of L-lysine,and the L-lysine production was improved effectively when adding acetic acid and Tween80." @default.
- W2358644310 created "2016-06-24" @default.
- W2358644310 creator A5035211538 @default.
- W2358644310 date "2009-01-01" @default.
- W2358644310 modified "2023-09-24" @default.
- W2358644310 title "Breeding of L-lysine Producing Mutant and Studying under Fermentation Conditions" @default.
- W2358644310 hasPublicationYear "2009" @default.
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