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- W2358647622 abstract "Objective The aim of this study was to explore the effects of two human ovarian tissue cryopreservation protocols for preserving germ cells.Methods Biopsies of ovarian tissue were collected from total 15 patients after informed consents.The tissues were randomly allocated to fresh,vitrification and conventional slow freezing groups.Morphological integral primordial follicles rates were compared between fresh and the post warming/thawing tissue.Endocrinal functions of the post-cryopreservation tissue were also investigated and compared by estradiol and progesterone detections in in-vitro culture medium every other day.Results In this study,the morphological integral primordial follicles rates in the fresh,vitrification,and slow-freezing groups were 97.6%,80.3% and 72.6%,respectively.After warming/thawing,the rates significantly reduced in both freezing groups(P 0.001).No statistically significant differences in normal morphology primordial follicle rates were observed between the two cryopreservation approaches(P=0.237).During in-vitro culture,estradiol and progesterone were secreted continuously by the both post-cryopreservation tissue pieces.And there were no statistical differences between the two groups(P 0.05).Conclusions Our study demonstrates that the two methods for human ovarian tissues cryopreservation are effective and can be fitted for different requires." @default.
- W2358647622 created "2016-06-24" @default.
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- W2358647622 date "2006-01-01" @default.
- W2358647622 modified "2023-09-26" @default.
- W2358647622 title "Research of Two Methods for Human Ovarian Tissues Cryopreservation" @default.
- W2358647622 hasPublicationYear "2006" @default.
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