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- W2358649207 abstract "As an important nature immunological factor in Hyriopsis cumingii,alpha-2 macroglobulin plays necessary role in immunological defence and immunoregulation.In this study,α2M gene from Hyriopsis cumingii was cloned by RACE approach.The accession number in the Genbank is DQ 993 157.1.After systematic analysis through bioinformatics approaches,The results showed that the full-length cDNA spans 5 124 bp,which contains an open reading frame of 4 836 bp,5' untranslated region of 35 bp and 3'untranslated region of 253 bp.The open reading frame encodes a protein of 1 611 amino acids.The first 23 amino acids in the N-terminus are recognized as a signal peptide.The relative molecular weight of the deduced protein is 177 571.8 u,isoelectric point is 5.49,and instability index is 39.53,which is classified as stable.Based on the results above,expression of α2M in different tissues and physiological states of Hyriopsis cumingii was determined by relative quantitive RT-PCR,the internal standard gene is 18 S.The result showed no expression was detected in mantel,conductor muscle,intestine,sexual gland except in hemocyte.After injecting with Escherichia coli and Aeromonas hydrophila,α2M expression level had a certain amount of rise 12 h later,which fully proved the existence of α2M immune system in Hyriopsis cumingii.The disease-resistant gene cloning and functional study provide new theoretical foundation and technical support for Hyriopsis cumingii's immunology research and disease-resistant varieties of breeding." @default.
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- W2358649207 date "2008-01-01" @default.
- W2358649207 modified "2023-09-24" @default.
- W2358649207 title "Full-length cDNA cloning and expression characterization of alpha-2 macroglobulin from Hyriopsis cumingii" @default.
- W2358649207 hasPublicationYear "2008" @default.
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