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- W2358663285 abstract "Effects of thidiazuron(TDZ) on flowering of Dendrobium nobile were investigated,by spraying on leaves with a concentration of 20 mg / L; and to further investigate the molecular mechanism of the process,the VRN1-like gene was cloned from protocorm-like bodies(PLBs) of Dendrobium nobile,the effects of cytokinins on its expression in axillary buds and PLBs were studied using semi-quantitative RT-PCR analysis. The results showed that flowering of D. nobile could be induced by application with TDZ instead of cold; the full-length cDNA of a VRN1-like gene named DnVRNA(GenBank accession No. EF535598) was cloned,which encodes a peptide with 63% and 62% similarity to the VRN1 protein from Festuca arundinacea and Triticum monococcum,respectively. Semi-quantitative RT-PCR analysis showed that the expression of DnVRNA in axillary buds was improved by TDZ,and those in PLBs were also up-regulated by cytokinins and cold,which suggested that it would function in cytokinin-induced floral transition." @default.
- W2358663285 created "2016-06-24" @default.
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- W2358663285 date "2013-01-01" @default.
- W2358663285 modified "2023-09-24" @default.
- W2358663285 title "Cloning and Expression Analysis of a VRN1-like Gene from Dendrobium Nobile" @default.
- W2358663285 hasPublicationYear "2013" @default.
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