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- W2358668032 abstract "Objective To examine whether rabbit chondrocytes can be maintained in vitro over long time periods and to study the effect of basic fibroblast growth factor(bFGF)on the expression of p53 mRNA in cultured rabbit chondrocytes. Methods The rabbit articular chondrocytes were harvested enzymatically and cultured in Dulbecco's modified eagle's medium (DMEM) with 10ng/ml bFGF. The morphological assessment of chondrocytes of the rabbit joint in different passages was proceded by light microscopy. The level of p53 mRNA was examined by RT-PCR. Results The proliferation of chondrocytes increased with bFGF added into the culture with less apoptotic cells in bFGF group than in control group. bFGF could decrease the expression of p53 mRNA in passaged cultured chondrocytes, with the significant difference of the objective gene indexes of p53 compared with control (P0.05). Conclusion bFGF downregulates the expression of p53 mRNA in cultured rabbit chondrocytes." @default.
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- W2358668032 date "2007-01-01" @default.
- W2358668032 modified "2023-09-24" @default.
- W2358668032 title "The Effect of Basic Fibroblast Growth Factor on the Expression of p53 Genes in Cultured Rabbit Chondrocytes" @default.
- W2358668032 hasPublicationYear "2007" @default.
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