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- W2358703275 abstract "Objective:A simple,sensitive and rapid RP-HPLC method has been developed for the determination of troxipide in human serum.Methods:A Beckman 344 model HPLC instrument with Ultrasphere ODS(250 mm×4 6 mm,5 μm) was used.The mobile phase was a mixture of methanol and 0 01 mol·L -1 phosphate buffer (pH 2 5) in a ratio of 35∶65 at a flow rate of 1 0 mL·min -1 ,and the ultraviolet wavelength was set at 254 nm.Theophylline was used as internal standard.The serum was extracted by ethyl acetate.Results:The calibration curve was linear in the range of 0 03～4 0 mg·L -1 ( r =0 999 8),and the limit of quantification was 0 01 mg·L -1 .The extraction recovery was between 79 4% and 81 1%,and the methodological recovery was between 97 3% and 105 0%.The within-day RSD and inter-day RSD were less than 5% and 10%,respectively. Conclusion:This method is simple,sensitive and rapid.It has been used in pharmacokinetics and bioavailability study of troxipide." @default.
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- W2358703275 date "2001-01-01" @default.
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- W2358703275 title "RP-HPLC Determination of Troxipide in Human Serum" @default.
- W2358703275 hasPublicationYear "2001" @default.
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