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- W2358746813 abstract "Objective To established an RP-HPLC method to determine the biotransformation efficiency of oleanolic acid using 25 kinds of fungi.Methods The optimum HPLC conditions were as follows:column was Kromasil C18 column(250 mm×4.6 mm,5 μm),mobile phase was methanol-0.1% acetic acid solution(V∶V=94∶6),flow rate was 1.0 mL·min-1 and the detection wavelength was set at 210 nm.Results The calibration curve was A=6.778×106ρ-2.539×104 with the linear in the range of 10-80 mg·L-1 for oleanolie acid(r=0.999 8),and the results showed that oleanolic acid was biotransformed by five kinds of fungi with the transformation efficiency of 56.2%,69.7%,77.9%,81.6% and 83.0%,respectively.Conclusions This detective method is reasonable,stable and accurate for the screen by fungi and determination of the biotransformation efficiency of oleanolic acid." @default.
- W2358746813 created "2016-06-24" @default.
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- W2358746813 date "2010-01-01" @default.
- W2358746813 modified "2023-09-26" @default.
- W2358746813 title "Qualitative and quantitative determinations of the biotransformation products of oleanolic acid" @default.
- W2358746813 hasPublicationYear "2010" @default.
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