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- W2358757635 abstract "ObjectiveTo establish the rapid detection of resistance to isoniazid in Mycobacterium tuberculosis by membrane oligonucleotide probes-reverse dot blot hybridized technique.MethodsPrepared the oligonucleotide probes of isoniazidresistant genes(katG,inhA) and drop it on nitrocellulose membrane.The target DNA fragments of M.tuberculosis clinical isolates were labeled with biotin by PCR amplification,and then hybridized with oligonucleotide probes on membrane.Polymerase chain reaction-Single stranded conformation polymorphism(PCR-SSCP) and PCR-direct sequencing(PCR-DS) techniques were used as the control.ResultsOf 20 isoniazid-sensitive strains,9 strains showed hybridization with probe K1 as H37Rv,10 sensitive strains showed positive hybridization with K1b',PCR-DS showed the AGC→ACC mutation at codon 315 of katG gene,1 strains showed positive hybridization with K1c',PCR-DS showed the AGC→AAC mutation at codon 315 of katG gene;15 strains showed positive hybridization with probe inh1 as H37Rv,5 strains with Inha1,PCR-DS showed the C→T mutation at-15 site of inhA gene;Of 36 isoniazid-resistant strains,17 strains showed positive hybridization with probe K1 as H37Rv;18 strains with probe K1b',1 strains showed negative hybridization,PCR-DS showed the GGC→GAC mutation at codon 279 of katG gene;25 strains showed positive hybridization with probe inh1,11 strains with Inha1.Mutation rate was(50 %) and(30.65 %) respectively.ConclusionThe membrane oligonucleotide probes-reverse dot blot hybridized technique was simple and rapid and could be used to detect isoniazid-resistance of partial Mycobacterium tuberculosis clinical strains." @default.
- W2358757635 created "2016-06-24" @default.
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- W2358757635 date "2006-01-01" @default.
- W2358757635 modified "2023-09-24" @default.
- W2358757635 title "RAPID DETECTION OF RESISTANT GENE MUTATION TO ISONIAZID IN MYCOBACTERIUM TUBERCULOSIS BY MEMBRANE OLIGONUCLEOTIDE PROBES HYBRIDIZATION" @default.
- W2358757635 hasPublicationYear "2006" @default.
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